JOVE JOURNAL

Immunology and Infection

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Journal (Immunology and Infection)

Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete

Diane G. Edmondson¹, Steven J. Norris¹, Bridget D. De Lay¹

¹University of Texas Health Science Center at Houston - McGovern Medical School

Journal (Immunology and Infection)

Establishing and Maintaining Sf1Ep Cell Stocks for Treponema pallidum Cultivation

Diane G. Edmondson¹, Steven J. Norris¹, Bridget D. De Lay¹

¹University of Texas Health Science Center at Houston - McGovern Medical School

Journal (Immunology and Infection)

In Vitro Cultivation of Treponema pallidum in Co-Culture with Sf1Ep Cells

Diane G. Edmondson¹, Steven J. Norris¹, Bridget D. De Lay¹

¹University of Texas Health Science Center at Houston - McGovern Medical School

Journal (Immunology and Infection)

Generation of Human Chimeric Antigen Receptor Regulatory T Cells

Russell W. Cochrane^1,2,3, Rob A. Robino^1,2,3, Leonardo M. R. Ferreira^1,2,3

¹Department of Microbiology and Immunology,Medical University of South Carolina, ²Department of Regenerative Medicine and Cell Biology,Medical University of South Carolina, ³Hollings Cancer Center,Medical University of South Carolina

Journal (Immunology and Infection)

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Kathryn M. Crotty^1,2, Samantha M. Yeligar^1,2

¹Department of Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine,Emory University, ²Atlanta Veterans Affairs Health Care System

Journal (Immunology and Infection)

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Gabriel S. Oliveira^1,2, Clara M. Bento^1,3,4, António Pombinho^1,3, Rita Reis^1,3, Kevin Van Calster^5,6, Linda De Vooght⁵, André F. Maia^1,3, Paul Cos⁵, Maria Salomé Gomes^1,2, Tânia Silva^1,2

¹i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, ²ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, ³IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, ⁴Programa Doutoral em Biologia Molecular e Celular (MCBiology),Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, ⁵Laboratory for Microbiology,Parasitology and Hygiene (LMPH), ⁶Department of Infectious Diseases in Humans, Immune Response,Sciensano

Journal (Immunology and Infection)

Antimicrobial Activity and Toxicity Assessment of Compounds Targeting Mycobacterium abscessus in Macrophage Infection Assays

Gabriel S. Oliveira^1,2, Clara M. Bento^1,3,4, António Pombinho^1,3, Rita Reis^1,3, Kevin Van Calster^5,6, Linda De Vooght⁵, André F. Maia^1,3, Paul Cos⁵, Maria Salomé Gomes^1,2, Tânia Silva^1,2

¹i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, ²ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, ³IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, ⁴Programa Doutoral em Biologia Molecular e Celular (MCBiology),Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, ⁵Laboratory for Microbiology,Parasitology and Hygiene (LMPH), ⁶Department of Infectious Diseases in Humans, Immune Response,Sciensano

Journal (Immunology and Infection)

In Vitro Differentiation of Naive CD4⁺ T Cells into Pathogenic Th17 Cells in Mouse

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

Isolation and Preparation of Mice Splenic Single-Cell Suspension for Flow Cytometry Analysis

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

Purification and Analysis of Mice Naive CD4⁺ T-Cells Using Magnetic Bead-Based Negative Selection

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

In Vitro Induction of Pathogenic Th17 Cells into Naive CD4⁺ T-Cells of Mice

Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng^1,2, Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine,Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine,Southeast University

Journal (Immunology and Infection)

Imaging Initial Ca²⁺ Microdomains in Primary T Cells

Fynn Gerlach*¹, Franziska Möckl*¹, Dejan Kovacevic^1,2,3, Valerie J. Brock¹, Riekje Winzer⁴, Lina Meyer^2,3, David Lohr^2,3, Lena-Marie Woelk^2,3, Eva Tolosa⁴, René Werner^2,3, Björn-Philipp Diercks¹

¹The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology,University Medical Centre Hamburg-Eppendorf, ²Department of Applied Medical Informatics,University Medical Centre Hamburg-Eppendorf, ³Department of Computational Neuroscience,University Medical Centre Hamburg-Eppendorf, ⁴Department of Immunology,University Medical Centre Hamburg-Eppendorf

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